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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Propidium monoazide ： ウィキペディア英語版 Propidium monoazide Propidium monoazide (PMA) is a photoreactive DNA-binding dye that preferentially binds to dsDNA. It is used to detect viable microorganisms by qPCR. Visible light (high power hallogen lamps or specific LED devices) induces a photoreaction of the chemical that will lead to a covalent bond with PMA and the dsDNA. This will render the DNA non-amplifiable by PCR. Dead microorganisms lose their capability to maintain their membranes, which leaves the "naked" DNA in the cytosol ready to react with PMA. Living organisms don't react to the PMA, as they have an intact cell membrane. After treatment with the chemical, only the DNA from living bacteria is usable in qPCR, allowing to obtain only the amplified DNA of living organisms. This is helpful in determining which pathogens are active in specific samples in. The main use of PMA is in Viability PCR but the same principle can be applied in flow cytometry or fluorescence microscopy. ==References== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Propidium monoazide」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.